Active participation in lectures. Successful completion of written tests by current classification scale for at least 56 %. The final grade will be determined in accordance with the rules set out in the STU study regulations.

Student masters the principles of most methods used in metabolomics – NMR spectroscopy including in vivo, and mass spectrometry with chromatography separation. He/she knows how to use it studying endo- and exo-metabolome with their respective advantages and disadvantages. The graduate also knows how to evaluate data using multivariate statistical methods.

The introduction to metabolomics. NMR spectroscopy - principles, basic parameters, NMR spectrometer. Sample preparation and measuring NMR spectra. Structure determination and methods of metabolite quantification.
In vivo MRS. Animal models, localization. Advanced MRS methods.
GC/MS and LC/MS. Separation principles. Sample preparation. Derivatization. Ways of ionization and types of detectors. Data evaluation.
Statistical evaluation of metabolomic data – one and multidimensional analysis, data preparation, PCA, PLS-DA