Course unit code:
Course unit title:
Mode of delivery, planned learning activities and teaching methods:
lecture – 2 hours weekly (on-site method)
Credits allocated:
Recommended semester:
Food, Hygiene, Cosmetics – master (full-time, attendance method), 1. semester
Biotechnology – master (full-time, attendance method), 1. semester
Biochemistry and Biomedical Technologies – master (full-time, attendance method), 1. semester
Nutrition and Food Quality Assessment – master (full-time, attendance method), 1. semester
Level of study:
Assesment methods:

Active participation in lectures. Successful completion of written tests by current classification scale for at least 56 %. The final grade will be determined in accordance with the rules set out in the STU study regulations.

Learning outcomes of the course unit:

Student masters the principles of most methods used in metabolomics – NMR spectroscopy including in vivo, and mass spectrometry with chromatography separation. He/she knows how to use it studying endo- and exo-metabolome with their respective advantages and disadvantages. The graduate also knows how to evaluate data using multivariate statistical methods.

Course contents:

The introduction to metabolomics. NMR spectroscopy - principles, basic parameters, NMR spectrometer. Sample preparation and measuring NMR spectra. Structure determination and methods of metabolite quantification.
In vivo MRS. Animal models, localization. Advanced MRS methods.
GC/MS and LC/MS. Separation principles. Sample preparation. Derivatization. Ways of ionization and types of detectors. Data evaluation.
Statistical evaluation of metabolomic data – one and multidimensional analysis, data preparation, PCA, PLS-DA

Language of instruction:
Name of lecturer(s):
Ľ. Horanská, N. Krivoňáková, Z. Takáč (2023/2024 – Winter)
Course supervisor:
prof. Ing. Ivan Špánik, DrSc.
Last modification:
28. 11. 2023

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