Student masters the principles of most methods used in metabolomics – NMR spectroscopy including in vivo, and mass spectrometry with chromatography separation. He/she knows how to use it studying endo- and exo-metabolome with their respective advantages and disadvantages. The graduate also knows how to evaluate data using multivariate statistical methods.

The introduction to metabolomics. NMR spectroscopy - principles, basic parameters, NMR spectrometer. Sample preparation and measuring NMR spectra. Structure determination and methods of metabolite quantification.
In vivo MRS. Animal models, localization. Advanced MRS methods.
GC/MS and LC/MS. Separation principles. Sample preparation. Derivatization. Ways of ionization and types of detectors. Data evaluation.
Statistical evaluation of metabolomic data – one and multidimensional analysis, data preparation, PCA, PLS-DA

Contact teaching: 26 h
lectures 23 h
measurement presentation and excursion 3 h

Contactless teaching: 24 h
preparation for lectures 12 h
preparation for tests 12 h

Four written tests from 4 main thematic parts make the final grade - Metabolomics and NMR spectroscopy (40 %), in vivo MRS (15 %), chromatography and mass spectrometry (15 %), statistical analysis (30 %). Evaluation according to the STU grading scale.