The introduction to metabolomics. NMR spectroscopy - principles, basic parameters, NMR spectrometer. Sample preparation and measuring NMR spectra. Structure determination and methods of metabolite quantification.
In vivo MRS. Animal models, localization. Advanced MRS methods.
GC/MS and LC/MS. Separation principles. Sample preparation. Derivatization. Ways of ionization and types of detectors. Data evaluation.
Statistical evaluation of metabolomic data – one and multidimensional analysis, data preparation, PCA, PLS-DA