Course unit code:
N418I0_4B
Course unit title:
Instrumental Methods in Analysis
Mode of completion and Number of ECTS credits:
Exm (4 credits)
Course contents:
1. Basic concepts and terms of analytical chemistry, the basics of evolution of analytical signal, priciples and use of instrumental methods of analysis. Electroanalytical, spectral and chromatographic methods of analysis, the overview of other methods. (allowance 2/2)
 
a. General aspects of analytical chemistry. The subject of analytical chemistryl. Analytical process as a concept of obtaining the analytical information. The classification and selection of analyticla methods. Qualitative requirements on chemical analysis. The basic rules of sampling, the ucertainty of sampling. The sample preparation. Analytical chemical measurements, the principles of analytical measurement. Signals in analytical chemistry. The characteristics of detectors. The signal and noise.
b. The tracing of measurements, adjusting, calibration and verification, regression and calibration with corresponding uncertainties, reference materials, standard sample, addition methods. The result of measurement (the accuracy, precision, errors and uncertainties of measuremen results). The evaluation of analytical information, information of quantitative analysis. The quality assurance in analytical chemistry, accreditation. Good laboratory praxis. Validation of methods. Statistical quality control. Analytical chemistry in technology and praxis.
c. Physical and physicochemical procedures for obtaining the analytical information. Electrochemical interactions as a basis of analytical signal. Electrochemical analytical methods, basic terms. Measurement of electrical quantities in chemical laboratory. Conductometry, Potentiometry - direct measurement of pH, pX. Potentiometric titrations. Addition methods. Potentiometric detectors.
d. Electrolysis and electrochemical reactions. Polarisation I-E curves. Electrogravimetry, coulometry with controlled potential, coulometric titrations. Voltammetry with constant surface area electrodes. Electrolysis under voltammetric conditions. Polarography, methods. Cyclic voltammetry. Sensitivity enhancement in voltammetry. Electrochemical stripping analysis. Voltammetric titrations. Amperometric and biamperometric titrations. Potentiometric titrations with polarised electrodes.
e. Chromatographic analysis - general aspects. The principles of separation of compounds, basic terms, the classification of chromatographic methods. Chemical interactions as a basis of chromatographic separation. The partitioning, adsorption, ionic, gel and affinity chromatography. Chromatogram, retention characteristics, retencion indexes. The peak pattern, the theory of chromatographic process, equations, resolution, separation efficiency.
f. The column chromatography. Experimental, qualitative and quantitative analysis. Liquid chromatography. Mobile and stationary phases. Sample introduction systems, detectors.
g. Gas chromatography, mobile and stationary phases, samplers. Temperature programs. Analytical use.
h. Thin layer and paper chromatography, stationary and mobile phases, the chromatogram deriving and evaluation, analytical use. Electromigration methods, electrophoresis, isotachophoresis, analytical use. The combined separation methods.
i. Interaction of electromagnetic radiation with analyte and the types of analytical signals. Emision of electromagnetic radiation as analytical information. Atomic emision spectrometry. Flame fotometry. ICP. Multielement spectral analysis. Luminescent analysis. Atomic fluorescence. Spectrometry of X-rays, Auger electron spectrometry, fotoelectron spectrometry, atomic mass spectrometry.
j. Analytical methods based on absorption of electromagnetic radiation. Atomic absorption spectrometry. Molecular absorption spectrometry (UV/VIS). Infrared spectrometry, Raman spectrometry. Absorption of electromagnetic radiation under concurent effect of magnetic field, NMR and EPR spectrometry. Mass spectrometry of organic compounds. The combination of methods of molecular spectrometry.
k. Analytical methods based on light scattering. Turbidimetry and nefelometry. Chiroptical methods - polarimetry and spectropolarimetry. Refractometry and interferometry. Radioanalytical methods, activation analysis.
l. Bioanalytical methods. Flow analysis, combined methods. Obtaining the analytical information for technology, process analysis. The specific application of analytical chemistry in praxis - clinical analysis, environmental analysis (water, sludge, sediment, soil analysis, atmospheric pollution analysis), foods analysis, analysis for metal processing and inorganic amd organic technology.

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