On-Line Monitoring of Biopolymer Content in Bacterial Cells Using Radio-Frequency Impedance
P. Hrnčiřík, J. Vovsík, V. Jurníček, J. Náhlík
Institute of Chemical Technology, Prague
Abstract
This paper describes the implementation of a radio-frequency (RF) impedance-based sensor for on-line monitoring of a biopolymer - medium chain length poly(3-hydroxyalkanoates) (mcl-PHAs, shortly PHA) production process by the Pseudomonas putida KT2442 strain with emphasis on on-line in-situ monitoring of PHA content in bacterial cells. First, motivation for the application of the commercially available RF impedance-based sensor technology is presented. Subsequently, experimental results obtained from fed-batch cultivations of the Pseudomonas putida KT2442 strain are presented and discussed, and a novel scheme for on-line estimation of the PHA content in bacterial cells under the conditions of aerated cultivations combining the capacitance and offgas composition measurements is proposed.
Full paper
Session
Process Measurements and Devices (Poster)
Reference
Hrnčiřík, P., Vovsík, J., Jurníček, V., Náhlík, J.: On-Line Monitoring of Biopolymer Content in Bacterial Cells Using Radio-Frequency Impedance. Editors: Fikar, M., Kvasnica, M., In Proceedings of the 17th International Conference on Process Control ’09, Štrbské Pleso, Slovakia, 254–258, 2009
BibTeX
| @inProceedings{pc09-022, | ||
| author | = { | Hrnčiřík, P. and Vovsík, J. and Jurníček, V. and Náhlík, J.}, |
| title | = { | On-Line Monitoring of Biopolymer Content in Bacterial Cells Using Radio-Frequency Impedance}, |
| booktitle | = { | Proceedings of the 17th International Conference on Process Control '09}, |
| year | = { | 2009}, |
| pages | = { | 254-258}, |
| editor | = { | Fikar, M. and Kvasnica, M.}, |
| address | = { | Štrbské Pleso, Slovakia}, |
| publisher | = { | Slovak University of Technology in Bratislava}, |
| url | = { | http://www.kirp.chtf.stuba.sk/pc09/data/papers/022.pdf}} |